high performance liquid chromatography hplc  (Bio-Rad)

Journal: Cardiology Research

Article Title: Analytical Evaluation of a Point-of-Care Platform for Glycated Hemoglobin and N-Terminal Pro-B-Type Natriuretic Peptide Testing in Cardiometabolic Care

doi: 10.14740/cr2220

Figure Lengend Snippet: Method comparison between AFIAS-3 POCT and Tosoh G8 HPLC for HbA1c. (a) Passing–Bablok regression showing strong correlation (r = 0.98, P < 0.0001; slope = 0.95; intercept = 0.32%). The blue area indicates the 95% confidence interval. (b) Bland–Altman plot showing a negligible mean bias of −0.03% (blue line) with 95% limits of agreement (red lines) ranging from −0.60% to +0.53%. HbA1c: hemoglobin A1c; POCT: point-of-care testing; HPLC: high-performance liquid chromatography.

Article Snippet: Comparators were: 1) HbA1c: Tosoh G8 high-performance liquid chromatography (HPLC) (NGSP-certified, IFCC-traceable); 2) NT-proBNP: cobas 8000 module e602 (Roche Diagnostics), electrochemiluminescence immunoassay (ECLIA).

Techniques: Comparison, High Performance Liquid Chromatography

Journal: Journal of Applied Glycoscience

Article Title: Structural Analysis of a Novel Trisaccharide Containing Fructopyranoside Residue Isolated from a Fermented Beverage of Plant Extracts

doi: 10.5458/jag.7302201

Figure Lengend Snippet: Super Ohtaka ® was loaded on to a carbon-Celite ® column and successively eluted with 30 % ethanol. The following operating conditions were used for HPLC: (a) column, Amide-80 (4.6 × 250 mm; Tosoh Corporation); column temperature, 80 °C; elution with 80 % acetonitrile at a flow rate of 1 mL/min; detection, by refractive index; (b) column, ODS-100V (4.6 × 250 mm × 2; Tosoh Corporation); column temperature, room temperature (approx. 25 °C); elution with distilled water at a flow rate of 0.5 mL/min; detection by refractive index.

Article Snippet: The 30 % ethanol fraction, which was suspected to contain unidentified oligosaccharides, was concentrated in vacuo and freeze-dried to give 2.65 g. This fraction was eluted with 80 % acetonitrile applied at a flow rate of 1.0 mL/min at 80 °C using a high-performance liquid chromatography (HPLC) system (Tosoh Corporation, Tokyo, Japan) equipped with an Amide-80 column (4.6 mm × 25 cm, Tosoh Corporation) and refractive index detection.

Techniques: Refractive Index

Journal: Journal of Applied Glycoscience

Article Title: Structural Analysis of a Novel Trisaccharide Containing Fructopyranoside Residue Isolated from a Fermented Beverage of Plant Extracts

doi: 10.5458/jag.7302201

Figure Lengend Snippet: Analysis of Saccharide 1 produced during fermentation was performed using high-performance anion exchange chromatography (HPAEC). Plant extract was fermented for: (a) 0 or (b) 180 days. Super Ohtaka ® (100 mL) fermented for 0 or 180 days was added to activated carbon (10 g), stirred for 3 h, and filtered. The activated carbon was extracted three times with 500 mL of 30 % ethanol. Ethanolic extracts were combined, concentrated to dryness, and solubilized in 1 mL of distilled water. Saccharide solutions were analyzed by HPAEC.

Article Snippet: The 30 % ethanol fraction, which was suspected to contain unidentified oligosaccharides, was concentrated in vacuo and freeze-dried to give 2.65 g. This fraction was eluted with 80 % acetonitrile applied at a flow rate of 1.0 mL/min at 80 °C using a high-performance liquid chromatography (HPLC) system (Tosoh Corporation, Tokyo, Japan) equipped with an Amide-80 column (4.6 mm × 25 cm, Tosoh Corporation) and refractive index detection.

Techniques: Produced, Chromatography, Plant Extract